Blocking by histones of accessibility to DNA in chromatin: addition of histones.
نویسندگان
چکیده
The blocking effect of histones on accessibility of DNA to DNase has been studied by addition of various histones to chromatin in intact or histone-depleted thymus nuclei (those from which all lysine-rich histone was removed and those from which much of the arginine-rich histone was removed) and to deoxyribonucleoprotein (chromatin) extracted from nuclei. In each case lysine-rich histone, weight for weight, blocks accessibility more effectively than does arginine-rich histone. Fractions of the arginine-rich histone differ considerably from each other in their blocking efficacy. Phosphorylation of lysine-rich histone tempers its blocking effectiveness.
منابع مشابه
Blocking by histones of accessibility to DNA in chromatin.
The effect of histones on accessibility of DNA to DNase in chromatin of thymus nuclei has been studied by selective extraction of either lysine-rich or arginine-rich histones. It was found that all histones block accessibility but that, weight for weight, lysine-rich histones block much more effectively than do arginine-rich histones. We point to the contrast between accessibility of DNA to DNa...
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 69 11 شماره
صفحات -
تاریخ انتشار 1972